Am Coo (ATCC® CRL-1286)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 6 months
Gender male
Clinical Data
The tissue donor suffered from osteogenesis imperfecta (tarda), and was the son of Ja Coo (ATCC CRL-1294) and brother of Pa Coo (ATCC CRL-1293).
male
Comments
The tissue donor suffered from osteogenesis imperfecta (tarda), and was the son of Ja Coo (ATCC CRL-1294) and brother of Pa Coo (ATCC CRL-1293).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.