Os Te (ATCC® CRL-1262)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age fetus
Gender female
Clinical Data
The tissue donor suffered from osteogenesis imperfecta congenita (severe, broad bone type).
female
Comments
The tissue donor suffered from osteogenesis imperfecta congenita (severe, broad bone type).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cross References

Nucleotide (GenBank) : U50330 Human procollagen C-proteinase (pCP-2) mRNA, complete cds.

Nucleotide (GenBank) : AH003197 Human (CRL 1262 variant allele) pro-alpha-1 type-I collagen gene.

Nucleotide (GenBank) : K03179 Human (CRL 1262 normal allele) alpha-1 type-I collagen gene, exon 26.

Nucleotide (GenBank) : K03176 Human (CRL 1262 variant allele) pro-alpha-1 type-I collagen gene, exons 31-30.

Nucleotide (GenBank) : K03178 Human (CRL 1262; normal allele) pro-alpha-1 type-I collagen gene, exons 31-27.

Nucleotide (GenBank) : K03177 Human (CRL 1262 variant allele) pro-alpha-1 type-1 collagen gene, exon 26, partial.