La Bel (ATCC® CRL-1179)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 47 years
Gender female
Applications
The cells exhibit hydroxylysine deficiency.
Clinical Data
The tissue donor suffered from type VI Ehlers-Danlos syndrome (hemorrhagic type), and had a deceased brother with same condition.
female
Comments
The tissue donor suffered from type VI Ehlers-Danlos syndrome (hemorrhagic type), and had a deceased brother with same condition.
The cells exhibit hydroxylysine deficiency.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation