WI-26 VA4 (ATCC® CCL-95.1)

Organism: Homo sapiens, human  /  Cell Type: SV40 transformed  / 

Organism Homo sapiens, human
Cell Type SV40 transformed
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age 3 month gestation
Gender male
Ethnicity Caucasian
Applications
The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Storage Conditions liquid nitrogen vapor temperature
Karyotype Chromosome Frequency Distribution 49 Cells: 2n = 46 Karyotype unstable within triploid stemline range of 67 to 71. Ninety-six percent of the cells have a large chromosome with a submedian centromere. Approximately 52% of the cells have one or more secondary constrictions; 42% of the cells examined had breaks.
Derivation
WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Clinical Data
male
Caucasian
3 month gestation
Comments
WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 9,11
D5S818: 11,12
D7S820: 10,13
THO1: 6,7
TPOX: 10,11
vWA: 17,20
Isoenzymes
G6PD, B
Name of Depositor AJ Girardi
References

Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659

Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085

Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

Basic Documentation
References

Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659

Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085

Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.