intestine 407 (ATCC® CCL-6)

Organism: Homo sapiens, human  /  Tissue: HeLa contaminant  / 

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Organism Homo sapiens, human
Tissue HeLa contaminant
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain human papilloma virus (HPV-18)]
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. HeLa Marker Chromosomes: One copy of M1, one copy of M2, four copies of M3 and one copy of M4 as revealed by G-banding patterns.
Derivation
This line was originally thought to be derived from normal embryonic intestinal tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
Clinical Data
female
HeLa Markers Y
Genes Expressed
keratin
Cellular Products
keratin
Tumorigenic Yes
Effects
Yes, in hamster (untreated) cheek pouch
Yes, in immunosuppressed rats
Virus Susceptibility Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Human adenovirus 3
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human coxsackievirus B1
Human Coxsackievirus B 2
Human Coxsackievirus B3
Human Coxsackievirus B 4
Human Coxsackievirus B 5
Human Coxsackievirus B 6
Mumps virus
Virus Resistance
rabiesvirus
Comments

The cells are positive for keratin by immunoperoxidase staining
Cells contain human papilloma virus (HPV-18).

Complete Growth Medium Basal medium (Eagle) in Earle's BSS, 90%; fetal bovine serum, 10%
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor G Henle, F Deinhardt
Year of Origin January, 1955
References

Henle G, Deinhardt F. The establishment of strains of human cells in tissue culture. J. Immunol. 79: 54-59, 1957. PubMed: 13475808

Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 14: 469-475, 1978. PubMed: 566722

Lavappa KS, et al. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 259: 211-213, 1976. PubMed: 1250349

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Henle G, Deinhardt F. The establishment of strains of human cells in tissue culture. J. Immunol. 79: 54-59, 1957. PubMed: 13475808

Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 14: 469-475, 1978. PubMed: 566722

Lavappa KS, et al. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 259: 211-213, 1976. PubMed: 1250349

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293