L-132 (ATCC® CCL-5)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain Human papillomavirus
Applications
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
Derivation
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers Y
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes. Remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor EV Davis
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996

. . Fed. Proc. 19: 386, 1960.

. . Science 133: 2059, 1961.

Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996

. . Fed. Proc. 19: 386, 1960.

. . Science 133: 2059, 1961.

Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993