LS123 (ATCC® CCL-255™)

Organism: Homo sapiens, human  /  Tissue: colon  /  Cell Type: epithelial

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Organism Homo sapiens, human
Tissue colon
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type B, colorectal adenocarcinoma
Age 65 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 63; range = 54 to 70.
The cells consistently have 18 to 20 marker chromosomes. They are: (t(1;?), del(1)(p13), del(2) (p11), t(2;?), t(7p, 19p), t(8;?), t(11;11) (p15;q13), t(14q,21q), t(6p,16p), t(X;?), and 10-12 others. There is only one copy per cell of structurally unchanged normal chromosomes for Nos. 1,2, 4, 10, 14, 16, and 18. No. 20 generally has 4 copies per cell and the X has 3 copies per cell. No Y chromosome was detected.
Images
Derivation
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The epithelial cell line, LS123, was established by L.P. Rutzky et al, in 1974 from the second in a series of three primary colonic tumors resected from a 65 year old Caucasian female. 

In 1950, a primary colon tumor was resected from the patient (41 years old at that time). 24 years later a second primary tumor classified as a Dukes' type B mucin producing colon adenocarcinoma was excised and the cells were cultured.

A third primary tumor was discovered in 1980 in an area just proximal to the colostomy and was resected.
Clinical Data
65 years
Caucasian
female
Genes Expressed
carcinoembryonic antigen (CEA), 9 ng/mL per 106 cells per 10 days; mucin
Tumorigenic No
Effects
No, in nude mice
(Tumors did not develop within three months in nude mice (5/5) inoculated subcutaneously with 10(7) cells.)
Comments

The established cell line is aneuploid, releases small amounts of carcinoembryonic antigen, fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells.

LS123 cells grow as confluent cell monolayers in either low serum or serum free medium.

In the chick embryonic skin assay, the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness.

Although LS123 exhibits several properties associated with neoplasia, they have little invasive potential and may represent an early stage in tumor development.

The plating efficiency is 12% in the medium recommended below.

This cell line is non-tumorigenic in mice. Tumors did not develop within three months in nude mice (5/5) inoculated subcutaneously with 107 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 9,13
D16S539: 13
D5S818: 11
D7S820: 9
THO1: 7,8
TPOX: 9
vWA: 16,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor LP Rutzky
Year of Origin March, 1974
References

Rutzky LP, et al. Characterization of a human colonic adenocarcinoma cell line, LS 123. In Vitro 19: 99-107, 1983. PubMed: 6826200

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer Cell Line Mutation Data Other Document
Cell Micrograph Other Document
p53 Hotspot Mutation Data
References

Rutzky LP, et al. Characterization of a human colonic adenocarcinoma cell line, LS 123. In Vitro 19: 99-107, 1983. PubMed: 6826200