LL 86 (LeSa) (ATCC® CCL-190)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: sarcoma

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease sarcoma
Age 18 years
Gender male
Ethnicity Caucasian
Applications
The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
Karyotype normal human male; diploid
Derivation
The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
Clinical Data
The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
male
Caucasian
18 years
Comments
The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
Complete Growth Medium Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse the monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Isoenzymes
G6PD, B
Name of Depositor RG Crystal