CCRF-SB [CCRF SB] (ATCC® CCL-120™)

Organism: Homo sapiens, human  /  Tissue: peripheral blood  /  Cell Type: B lymphoblast

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells contain Herpesvirus]
Disease acute lymphoblastic leukemia
Age 11.5 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

Derived from a buffy coat preparation from a patient with acute lymphoblastic leukemia.

A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
Clinical Data
11.5 years
Caucasian
male
Antigen Expression
HLA A1, A2, B12, B17, Cw2
Comments The cells are EBNA positive
Complete Growth Medium RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 105 viable cells/mL. 
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
Cryopreservation culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
Isoenzymes
G6PD, B
Name of Depositor GE Foley
References

Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067

Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067

Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592