R1E/TL8x.1 (ATCC® TIB-43)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain C58/J
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
H-2k; Thy-1.2
Genes Expressed
H-2k; Thy-1.2
Comments
Unlike the parental line R1.1, these cells do not express TL antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor R Hyman
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

derived from R1.1 (ATCC TIB-42).

Basic Documentation
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

derived from R1.1 (ATCC TIB-42).