Swiss SFME (Serum Free Mouse Embryo) (ATCC® CRL-9391)

Organism: Mus musculus, mouse  /  Cell Type: Astrocyte  / 

Organism Mus musculus, mouse
Cell Type Astrocyte
Product Format frozen
Morphology fibroblast
Culture Properties clusters in suspension; the cells will attach to flasks coated with 0.02 mg/ml human fibronectin
Biosafety Level 1
Age embryo; 16 days
Strain Swiss
Applications
This line was derived from minced, trypsinized Swiss mouse embryos grown in serum free medium.
Karyotype modal number = 40; range = 39 to 42
Derivation
This line was derived from minced, trypsinized Swiss mouse embryos grown in serum free medium.
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
This line was derived from minced, trypsinized Swiss mouse embryos grown in serum free medium.
BALB/c SFME cells infrequently form colonies in soft agar and are not tumorigenic if injected into nude mice.
Either serum or TGF-beta induce astrocyte differentiation accompanied by GFAP (glial fibrillary acidic protein) expression. Furthermore, the presence of serum causes cell growth arrest.
This process is reversible upon removal of the serum.
Swiss SFME cells do not grow in conventional media supplemented with fetal bovine serum or bovine calf serum.
When cultured in the serum free medium (see below), Swiss SFME cells reportedly can be propagated for extended periods without undergoing"crisis" or gross chromosomal aberration.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50%; Ham's F12 medium, 50%, Supplements: 0.01 mg/ml bovine insulin, 0.025 mg/ml human transferrin, 0.02 mg/ml human high density lipoprotein (HDL), 100 ng/ml mouse epidermal growth factor (EGF), 10 nM sodium selenite, 1 unit/ml human platelet derived growth factor (PDGF), and 15 mM HEPES. NOTE: HDL is available from Sigma Chemical Co. (catalog number L2014).
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, and collect suspended clusters by centrifugation.
To disperse clusters or to remove adherent cells, add fresh 0.25% Trypsin, 0.02% EDTA at room temperature until the cells disperse (2 to 3 minutes).
Add an equal volume of fresh serum free medium plus 0.1% soybean trypsin inhibitor and centrifuge at 1000 X g.
Resuspend the cell pellet in growth medium, aspirate and dispense into new (fibronectin coated, if adherence is desired) flasks.
Floating clusters may also be remove using a pipette and transferred to a flask containing fresh growth medium.
Cryopreservation
culture medium, 80%; fetal bovine serum, 10%; DMSO, 10%
Basic Documentation
References

Loo DT, et al. Extended culture of mouse embryo cells without senescence: inhibition by serum. Science 236: 200-202, 1987. PubMed: 3494308