BM-N (ATCC® CRL-8910)

Organism: Bombyx mori, silkworm  / 

Permits and Restrictions

View Permits

Organism Bombyx mori, silkworm
Product Format frozen
Culture Properties mixed: adherent and suspension
Biosafety Level 1
Applications
transfection host
Host suitable for infection by Bombyx mori nuclear polyhedrosis virus (BmNPV), and may be used to propagate BmNPV-based expression vectors.
Comments
The line should be cultivated at 27C.
Complete Growth Medium The base medium for this cell line is IPL-41 Insect Cell Culture Medium with glutamine (available from Sigma or Gibco). To make the complete growth medium, add the following components to the base medium:
  • 2.6 g/L Tryptose Phosphate Broth (TPB)
  • 0.069 mg/L ZnSO4·7H2O
  • 3.86 mg/L AlCl3·6H2O
  • 5 mg/L chlorophenol red
  • 2g/L NaCl
  • 10% heat-inactivated insect grade fetal bovine serum

  • Adjust pH to 6.3 to 6.4 by adding 1N NaOH
    Adjust osmolarity to 317 to 360 by adding extra NaCl

    The IPL-41 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation. (In Vitro 17: 495-509, 1981)
Subculturing
Medium Renewal: Once per week
Gently resuspend attached cells in old culture medium by pipetting old medium across the cells on the floor of the flask.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 27.0°C
Name of Depositor Daiichi Seiyaku Co., Ltd.
References

Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694

. . J. Seric. Sci. Jpn. 53: 547-548, 1984.

Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992

Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694

. . J. Seric. Sci. Jpn. 53: 547-548, 1984.

Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992

Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992