EJG (ATCC® CRL-8659)

Organism: Bos taurus, cow  /  Cell Type: endothelial  / 

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Organism Bos taurus, cow
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Applications
The line was derived from collagenase dissociated bovine adrenal tissue.
Derivation
The line was derived from collagenase dissociated bovine adrenal tissue.
Genes Expressed
Factor VIII:C
Comments
The line was derived from collagenase dissociated bovine adrenal tissue.
The cellular morphology is that of capillary endothelium.
The cells form linearly oriented confluent cultures.
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.1% EDTA and allow the flask to sit at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Seed new flasks (75 sq. cm.) with 1 X 10 exp4 cells.
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 110 hrs
Name of Depositor The United States of America
References

Ornberg R, et al. Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C. US Patent 4,670,394 dated Jun 2 1987

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
References

Ornberg R, et al. Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C. US Patent 4,670,394 dated Jun 2 1987

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438