CMH1a (ATCC® CRL-8399)

Organism: Mus musculus, mouse  /  Disease: Papilloma, hepatitis

Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Papilloma, hepatitis
Strain RIII
Applications
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
Derivation
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HeLa Markers N
Genes Expressed
surface antigen [sAg]
Comments
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
The construct contains the entire BPV genome and the hepatitis B sAg structural gene inserted within the MT gene between the promoter and the rest of the gene.
A BamHI/SalI fragment of pRF300 (containing hepatitis B sAg and MT-1 promoter) was ligated to a BamHI/SalI fragment of pB2-2 (containing the entire BPV genome and pBR sequences) to create pRB-1.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of DMEM, is ATCC catalog No. 30-2601.

Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense, into new flasks.
Cryopreservation
fetal bovine serum, 90%; glycerol, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Integrated Genetics, Inc.
References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987

Basic Documentation
References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987