Super Dome (ATCC® CRL-2286)

Organism: Canis familiaris  /  Cell Type: Epithelial,Epithelial-like  / 

Organism Canis familiaris
Cell Type Epithelial,Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Applications
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Storage Conditions liquid nitrogen vapor temperature
Derivation
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Clinical Data
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
female
Comments
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Super Dome forms domes that are approximately 5 times the area of MDCK.
For dome formation, the cells should be fed twice a day by a complete medium change.
Complete Growth Medium These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor RJ Klebe
References

Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715

Basic Documentation
References

Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715