NF-1 (ATCC® CRL-2075)

Organism: Mus musculus, mouse  /  Disease: nullipotent embryonal carcinoma; teratocarcinoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology epithelial
Culture Properties mixed adherent-suspension
Biosafety Level 1
Disease nullipotent embryonal carcinoma; teratocarcinoma
Age embryo; 7 days gestation
Gender male
Strain BALB/c
Applications
This pluripotent stem cell line was established in 1979 by Fox et al.
Derivation
This pluripotent stem cell line was established in 1979 by Fox et al.
Clinical Data
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
male
Tumorigenic Yes
Effects
Yes, when injected intramuscularly into syngeneic mice
Comments
This pluripotent stem cell line was established in 1979 by Fox et al.
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
This is a mixed cell culture; cells grow both as a monolayer and as a suspension. Subcultures are prepared by scraping the attached cell into the medium containing the floaters. Aspirate and dispense into new culture flasks. Subculture approximately two times weekly. Cells will detach from surface of flask before confluency is reached.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor I Damjanov
Year of Origin 1979
References

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898