NFPE (ATCC® CRL-2069)

Organism: Mus musculus, mouse  /  Disease: embryonal carcinoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease embryonal carcinoma
Age embryo; blastocyst
Strain BALB/c
Applications
The line was established from NF-1 mouse embryonal carcinoma established by Fox et al.
Phenotypically, the cells resemble primitive endoderm from 5 to 6 day mouse embryos.
Derivation
The line was established from NF-1 mouse embryonal carcinoma established by Fox et al. (see ATCC CRL-2075).
Comments
The line was established from NF-1 mouse embryonal carcinoma established by Fox et al. (see ATCC CRL-2075).
Phenotypically, the cells resemble primitive endoderm from 5 to 6 day mouse embryos.
They are reactive with the SSEA-3 monoclonal antibody.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 4 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor I Damjanov
References

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchow's Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898