DH82 (ATCC® CRL-10389)

Organism: Canis familiaris  /  Cell Type: Macrophage-like  /  Disease: malignant histiocytosis

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Organism Canis familiaris
Cell Type Macrophage-like
Product Format frozen
Morphology macrophage
Culture Properties adherent
Biosafety Level 1
Disease malignant histiocytosis
Age 10 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Clinical Data
dog
male
10 years
Comments
The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles.
They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors.
The cells do not produce IL-1.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor The United States of America
References

Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993

Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993

Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786