DoCl1 (S+L-) (ATCC® CCL-34.1)

Organism: Canis familiaris  /  Disease: sarcoma

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Organism Canis familiaris
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease sarcoma
Age adult
Gender female
Applications
The cells are positive for keratin by immunoperoxidase staining.
Clinical Data
female
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Comments
The cells are infected with defective Moloney sarcoma virus.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for 1 to 2 minutes, remove trypsin and let culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor PT Peebles
References

Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940

Peebles PT, et al. Murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine S + L-cells. Virology 70: 313-323, 1976. PubMed: 57666

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Peeples PT, et al. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology 67: 344-355, 1975. PubMed: 52940

Peebles PT, et al. Murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine S + L-cells. Virology 70: 313-323, 1976. PubMed: 57666