Human genomic DNA (PZ-HPV-7) (ATCC® CRL-2221D)

Organism: Homo sapiens, human  / 

Product Format frozen Approx. 10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction

Cell Line Description:

Epithelial cells cultured from normal tissue of the peripheral zone of the prostate from a 70-year-old Caucasian male were transfected with HPV-18 DNA. Incorporation of HPV-18 DNA was confirmed by polymerase chain reaction; specific amplification of a 160-base pair fragment of the HPV-18 E6-transforming region was noted. Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 oncoprotein of HPV. The cells are negative for prostate specific antigen (PSA).

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Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.