BC3A (ATCC® TIB-60)

Organism: Mus musculus, mouse  /  Tissue: spleen  /  Disease: leukemia

Organism Mus musculus, mouse
Tissue spleen
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leukemia
Strain BALB/c
Applications
This line was derived from a leukemia induced by B/T-L virus.
The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-).
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from a leukemia induced by B/T-L virus.
Antigen Expression
H-2d; Ia8
Genes Expressed
H-2d; Ia8
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
This line was derived from a leukemia induced by B/T-L virus.
The cells are negative for surface immunoglobulin (sIg-) and Thy-1.2 (Thy-1.2-).
Erythroid differentiation and hemoglobin synthesis are NOT inducible by DMSO.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.01 mM 2-mercaptoethanol, 90%; heat- inactivated fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
The cells may also be grown as ascites in syngeneic mice.
Inject 2 X 10 exp5 cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days).
Name of Depositor B Chesebro
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295

Basic Documentation
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295