M5/49.4.1 (ATCC® TIB-238)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Lymphoma

Permits and Restrictions

View Permits View Restrictions

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Thy-1
Cellular Products
immunoglobulin; monoclonal antibody; against Thy-1
Comments
Animals were immunized with a secondary mixed lymphocyte culture (C57BL/6 mouse anti irradiated BN rat lymphoma).
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not block CTL mediated cytotoxicity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL.
Interval: Maintain cell density between 5 X 104 and 5 X 105 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype rat IgG2a
Name of Depositor TA Springer
References

Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This line is subject to a license agreement between Harvard University and Boehringer Mannheim Biochemicals. Any use of the line for other than research purposes must be negotiated in advance with Harvard University. Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):

References

Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264