PC 61 5.3 [PC 61, PC 61.5.3] (ATCC® TIB-222)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties suspension
Biosafety Level 1
Strain OFA (B cell); BALB/c (myeloma)
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with the B6.1 mouse cytotoxic T cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin-2 (interleukin 2, IL-2) receptor (CD25)
Comments

The antibody blocks IL-2 binding and IL-2 dependent growth stimulation, and immunoprecipitates IL-2 receptors.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 5 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density).
Cryopreservation
Complete growth medium 92.5%; DMSO, 7.5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1 , IgG1
Name of Depositor M Nabholz
References

Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505

Zubler RH, et al. Activated B cells express receptors for, and proliferate in response to, pure interleukin-2. J. Exp. Med. 160: 1170-1183, 1984. PubMed: 6434689

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505

Zubler RH, et al. Activated B cells express receptors for, and proliferate in response to, pure interleukin-2. J. Exp. Med. 160: 1170-1183, 1984. PubMed: 6434689

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm