HNK-1 [HNK1, Leu7] (ATCC® TIB-200)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody also reacts with glycoproteins present on Schwann cells, oligodendrocytes and embryonic neurons.
Tested and found negative for ectromelia virus (mousepox).
The cells will not grow if the medium lacks 2-mercaptoethanol.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human natural killer (NK) cells and antigen dependent killer (K) cells (CD57)
Cellular Products
immunoglobulin; monoclonal antibody; against human natural killer (NK) cells and antigen dependent killer (K) cells (CD57)
Comments
Animals were immunized with a membrane extract of the human lymphoblastoid cell line HSB-2.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody also reacts with glycoproteins present on Schwann cells, oligodendrocytes and embryonic neurons.
Tested and found negative for ectromelia virus (mousepox).
The cells will not grow if the medium lacks 2-mercaptoethanol.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.02 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgM; kappa light chain
Name of Depositor T Abo, CM Balch
References

Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J. Immunol. 127: 1024-1029, 1981. PubMed: 6790607

Abo T, et al. Postnatal expansion of the natural killer and killer cell population in humans identified by the monoclonal HNK-1 antibody. J. Exp. Med. 155: 321-326, 1982. PubMed: 7054358

Schuller-Petrovic S, et al. A shared antigenic determinant between natural killer cells and nervous tissue. Nature 306: 179-181, 1983. PubMed: 6196639

McGarry RC, et al. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature 306: 376-378, 1983. PubMed: 6196641

Vincent M, Thiery JP. A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system. Dev. Biol. 103: 468-481, 1984. PubMed: 6202575

McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413

Tucker GC, et al. Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytes. Cell Differ. 14: 223-230, 1984. PubMed: 6207939

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J. Immunol. 127: 1024-1029, 1981. PubMed: 6790607

Abo T, et al. Postnatal expansion of the natural killer and killer cell population in humans identified by the monoclonal HNK-1 antibody. J. Exp. Med. 155: 321-326, 1982. PubMed: 7054358

Schuller-Petrovic S, et al. A shared antigenic determinant between natural killer cells and nervous tissue. Nature 306: 179-181, 1983. PubMed: 6196639

McGarry RC, et al. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature 306: 376-378, 1983. PubMed: 6196641

Vincent M, Thiery JP. A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system. Dev. Biol. 103: 468-481, 1984. PubMed: 6202575

McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413

Tucker GC, et al. Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytes. Cell Differ. 14: 223-230, 1984. PubMed: 6207939