M3/84.6.34 (ATCC® TIB-168)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
Tested and found negative for ectromelia virus (mousepox).
immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein),Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage.
Cellular Products
immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein)
Comments
Animals were immunized with detergent solubilized mouse(C57BL/6) macrophage membrane which had been depleted of previously identified antigens with monoclonal immunoadsorbants.
Like Mac-2, the Mac-3 antigen is not expressed on bone marrow cells (Mac-1 is expressed on bone marrow cells).
Also like Mac-2, Mac-3 appears to be expressed on their monocytic line of differentiation at a stage after divergence from the granulocytic series.
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.
Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype rat IgG1 kappa
Name of Depositor TA Springer
References

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):

References

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058