IGEL b4 (ATCC® TIB-141)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Tissue: spleen  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Cellular Products
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Comments
Animals were immunized with TNP-Asc.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition or replacement of fresh medium. Adherent cells can be dislodged by scraping and cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell culture density between 105 and 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions

Temperature: 37°C
Atmosphere: 5% CO

Isotype IgE (Igb haplotype)
Name of Depositor M Wabl
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Cross References

Nucleotide (GenBank) : X65775 M.musculus DNA for IgE antibody light chain (VJ).

Nucleotide (GenBank) : X65773 M.musculus DNA for IgE antibody heavy chain (VDJ).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.