M5/114.15.2 (ATCC® TIB-120)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Tissue: spleen  /  Disease: Lymphoma

Permits and Restrictions

View Permits View Restrictions

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Strain (BN x Lewis)F1
Storage Conditions liquid nitrogen vapor phase
Derivation
Rats were immunized with a secondary mixed lymphocyte culture (C57BL/6 mouse anti irradiated BN rat lymphoma).
Spleen cells were fused with NS-1 myeloma cells. 
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A b, I-A d, I-A q, I-E d and I-E k
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 viable cells/mL.
Medium Renewal: Every 2 to 4 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Isotype rat IgG2b kappa
Name of Depositor TA Springer
References

Bhattacharya A, et al. A shared alloantigenic determinant on 1a antigens encoded by the I-A and I-E subregions: evidence for 1 region gene duplication. J. Immunol. 127: 2488-2495, 1981. PubMed: 6170707

Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):RefBhattacharya A, et al. A shared alloantigenic determinant on 1a antigens encoded by the I-A and I-E subregions: evidence for 1 region gene duplication. J. Immunol. 127: 2488-2495, 1981. PubMed: 6170707

References

Bhattacharya A, et al. A shared alloantigenic determinant on 1a antigens encoded by the I-A and I-E subregions: evidence for 1 region gene duplication. J. Immunol. 127: 2488-2495, 1981. PubMed: 6170707

Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.