7C2C5C12 (ATCC® HB-8678)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain BALB/c
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Cellular Products
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum,
20%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor USDA
References

Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457