LS-125-2D4-11-10-1 [2D4] (ATCC® HB-12644)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody binds to the human CC-chemokine receptor 1 (CCR1).
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with the murine pre-B cell line L1-2 expressing high levels of transfected human CCR1. Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the human CC-chemokine receptor 1 (CCR1).
Comments
Animals were immunized with the murine pre-B cell line L1-2 expressing high levels of transfected human CCR1. Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the human CC-chemokine receptor 1 (CCR1).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1 ng/ml Interleukin-6 (IL-6), 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor Millennium Pharmaceuticals, Inc.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Qin S, et al. Anti-CCR1 antibodies and methods of use therefor . US Patent 6,329,510 dated Dec 11 2001

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Qin S, et al. Anti-CCR1 antibodies and methods of use therefor . US Patent 6,329,510 dated Dec 11 2001