TS 106 (ATCC® HB-12497)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts specifically with the human protein TS and displays negligible cross-reactivity with other cellular proteins found in human cells.
The antibody may be used in immunohistochemical, Western Blot, and ELISA assays to detect TS in human colon cell lines.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thymidylate synthase (TS)
Cellular Products
immunoglobulin; monoclonal antibody; against human thymidylate synthase (TS)
Comments
Animals were immunized with recombinant human thymidylate synthase (TS).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts specifically with the human protein TS and displays negligible cross-reactivity with other cellular proteins found in human cells.
The antibody may be used in immunohistochemical, Western Blot, and ELISA assays to detect TS in human colon cell lines.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Attached cells must be shaken loose or scraped.
Isotype mouse IgG1 kappa
Name of Depositor National Cancer Institute
References

Johnson PG, et al. Monoclonal antibodies specific for human thymidylate synthase. US Patent 6,221,620 dated Apr 24 2001

Johnson PG, et al. Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue. Cancer Res. 51: 6668-6676, 1992. PubMed: 1720706

Johnson PG, et al. Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines. Cancer Res. 52: 4306-4312, 1992. PubMed: 1643628

Edler D, et al. Thymidylate synthase expression: an independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer. Clin. Cancer Res. 6: 1378-1384, 2000. PubMed: 10778966

Edler D, et al. Immunohistochemical determination of thymidylate synthase in colorectal cancer--methodological studies. Eur. J. Cancer 33: 2278-2281, 1997. PubMed: 9470819

Edler D, et al. Immunohistochemically detected thymidylate synthase in colorectal cancer: an independent prognostic factor of survival. Clin. Cancer Res. 6: 488-492, 2000. PubMed: 10690528

Basic Documentation
References

Johnson PG, et al. Monoclonal antibodies specific for human thymidylate synthase. US Patent 6,221,620 dated Apr 24 2001

Johnson PG, et al. Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue. Cancer Res. 51: 6668-6676, 1992. PubMed: 1720706

Johnson PG, et al. Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines. Cancer Res. 52: 4306-4312, 1992. PubMed: 1643628

Edler D, et al. Thymidylate synthase expression: an independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer. Clin. Cancer Res. 6: 1378-1384, 2000. PubMed: 10778966

Edler D, et al. Immunohistochemical determination of thymidylate synthase in colorectal cancer--methodological studies. Eur. J. Cancer 33: 2278-2281, 1997. PubMed: 9470819

Edler D, et al. Immunohistochemically detected thymidylate synthase in colorectal cancer: an independent prognostic factor of survival. Clin. Cancer Res. 6: 488-492, 2000. PubMed: 10690528