CAB 117-12D10 [12D10] (ATCC® HB-10558)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender female
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen.
It is specific to amino acids 195-208 on the factor VII/VIIa molecule.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Clinical Data
female
Genes Expressed
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa)
Cellular Products
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa)
Comments
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen.
It is specific to amino acids 195-208 on the factor VII/VIIa molecule.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor Corvas, Inc.
U.S. Patent Number
References

Soule HR, Brunck TK. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex. US Patent 5,506,134 dated Apr 9 1996

Biemond BJ, et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb. Haemostasis 73: 223-230, 1995. PubMed: 7792734

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Soule HR, Brunck TK. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex. US Patent 5,506,134 dated Apr 9 1996

Biemond BJ, et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb. Haemostasis 73: 223-230, 1995. PubMed: 7792734