EL 1 (ATCC® CRL-9854)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology monocyte/macrophage
Culture Properties suspension
Biosafety Level 1
Applications
The EL 1 cell line was established from cryopreserved human spleen cells.
Derivation
The EL 1 cell line was established from cryopreserved human spleen cells.
Comments
The EL 1 cell line was established from cryopreserved human spleen cells.
The cells tend to grow as single cells but may form small nonadherent clusters.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml)
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor E.I. du Pont de Nemours & Co.
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995