90196B (ATCC® CRL-9853)

Organism: Homo sapiens, human  /  Tissue: spleen  / 

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Organism Homo sapiens, human
Tissue spleen
Product Format frozen
Morphology monocyte/macrophage
Culture Properties suspension
Biosafety Level 1
Storage Conditions liquid nitrogen vapor phase
Derivation
The 90196B cell line was established from cryopreserved human spleen cells.
Comments
The cells tend to grow in floating clusters.
Single cells may become adherent.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing The cells tend to grow in floating clusters. Single cells may become adherent. Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 x 105 and 1x106cells/mL. Gently pipette to break large clusters into smaller clusters. Medium Renewal: Twice weekly
Cryopreservation
Culture medium,85%; DMSO,10%; extra FBS,10%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Name of Depositor E.I. du Pont de Nemours & Co.
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995