LBRM-33-1A5 (ATCC® CRL-8079)

Organism: Mus musculus, mouse  /  Disease: lymphoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain B10.BR
Applications
The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Receptor Expression
interleukin 1 (IL-1)
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Complete Growth Medium RPMI 1640 medium with 0.0025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Maintain at approximately 8 X 10 exp5 viable cells/ml.
Name of Depositor S Gillis
References

Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. US Patent 4,404,280 dated Sep 13 1983

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. US Patent 4,404,280 dated Sep 13 1983