5A1 (ATCC® CRL-2702)

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Organism Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
ELISA detection colony stimulating factor 1 (macrophage)
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified mouse colony stimulating factor-1 (CSF-1). Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. The D24 (ATCC CRL-2701) antibody binds to mouse CSF-1 but does not neutralize its biological activity while the 5A1 (ATCC CRL-2702) antibody binds to and neutralizes the biological function of CSF-1 [PubMed: 2454994]. 5A1 binds both in vitro and in vivo [PubMed: 10792003]. The D24 antibody (ATCC CRL-2701) can be used with the 5A1 antibody (ATCC CRL-2702) in ELISA assays to quantitate the amount of naturally occurring mouse CSF-1 [PubMed: 2677146]. These antibodies recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1 [PubMed: 2677146]. The 5A1 antibody (ATCC CRL-2702) inhibits the clonal growth mouse granulocyte/macrophage colony forming cells (GM-CFC).
Genes Expressed
immunoglobulin; monoclonal antibody; against colony stimulating factor-1 (CSF-1),colony stimulating factor 1 (macrophage)
Cellular Products
immunoglobulin; monoclonal antibody; against colony stimulating factor-1 (CSF-1)
Tumorigenic Yes
Effects
Yes, ascites in pristane primed Lou/M rats
Comments
Animals were immunized with purified mouse colony stimulating factor-1 (CSF-1). Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. The D24 (ATCC CRL-2701) antibody binds to mouse CSF-1 but does not neutralize its biological activity while the 5A1 (ATCC CRL-2702) antibody binds to and neutralizes the biological function of CSF-1 [PubMed: 2454994]. 5A1 binds both in vitro and in vivo [PubMed: 10792003]. The D24 antibody (ATCC CRL-2701) can be used with the 5A1 antibody (ATCC CRL-2702) in ELISA assays to quantitate the amount of naturally occurring mouse CSF-1 [PubMed: 2677146]. These antibodies recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1 [PubMed: 2677146]. The 5A1 antibody (ATCC CRL-2702) inhibits the clonal growth mouse granulocyte/macrophage colony forming cells (GM-CFC).
Complete Growth Medium Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1
Name of Depositor H Lin
Year of Origin July, 1988
References

Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J. Immunol. 141: 483-488, 1988. PubMed: 2454994

Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J. Immunol. Methods 123: 123-129, 1989. PubMed: 2677146

Cenci S, et al. M-CSF neutralization and egr-1 deficiency prevent ovariectomy-induced bone loss. J. Clin. Invest. 105: 1279-1287, 2000. PubMed: 10792003

Basic Documentation
References

Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J. Immunol. 141: 483-488, 1988. PubMed: 2454994

Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J. Immunol. Methods 123: 123-129, 1989. PubMed: 2677146

Cenci S, et al. M-CSF neutralization and egr-1 deficiency prevent ovariectomy-induced bone loss. J. Clin. Invest. 105: 1279-1287, 2000. PubMed: 10792003