H3.1 (ATCC® CRL-2569)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Age adult
Strain BALB/c
Applications
ELISA detection zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
Western Blot zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
detection of zona pellucida glycoprotein 3 zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
zona pellucida glycoprotein 3
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Fetal bovine serum, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor J Dean
References

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547

Animals were immunized with a human zona pellucida 3 (ZP3) peptide with a C-terminal cysteine (RQPHVMSQWSRSASRNC) coupled to keyhole limpet haemocyanin. The zona pellucida is an extracellular matrix that mediates critical steps in fertilization and early development surrounds all vertebrate eggs, and in mice and humans comprises three glycoproteins: ZP1, ZP2 and ZP3.

Basic Documentation
References

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547

Animals were immunized with a human zona pellucida 3 (ZP3) peptide with a C-terminal cysteine (RQPHVMSQWSRSASRNC) coupled to keyhole limpet haemocyanin. The zona pellucida is an extracellular matrix that mediates critical steps in fertilization and early development surrounds all vertebrate eggs, and in mice and humans comprises three glycoproteins: ZP1, ZP2 and ZP3.