I-11.15 (ATCC® CRL-2470)

Organism: Mus musculus, mouse  /  Cell Type: macrophage  / 

Organism Mus musculus, mouse
Cell Type macrophage
Product Format frozen
Morphology macrophage
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Strain C3H/HeJ
Applications
This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse.
The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor.
This cell line, unlike I-13.35 (ATCC CRL-2471), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35.
The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses.
C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Derivation
This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse.
Clinical Data
female
Antigen Expression
CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +
Receptor Expression
colony stimulating factor 1 (CSF-1R, CD115)
Genes Expressed
CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +
Comments
This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse.
The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor.
This cell line, unlike I-13.35 (ATCC CRL-2471), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35.
The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer to new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor WS Walker
References

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081

McCormack JM, et al. Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell. Immunol. 145: 359-371, 1992. PubMed: 1451184

Basic Documentation
References

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081

McCormack JM, et al. Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell. Immunol. 145: 359-371, 1992. PubMed: 1451184