VSW (ATCC® CCL-129)

Organism: Vipera russelli, Russell's viper  / 

Permits and Restrictions

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Organism Vipera russelli, Russell's viper
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
The cells produce a C type retrovirus.
Karyotype model number = 64; range = 33 to 118. Karyotype slightly unstable with stemline number in hypertriploid range. Cells with modal numbers of 28 and 29 macrochromosomes and 34 to 37 microchromosomes. Many cells with a very small chromosome with a median centromere included with macrochromosomes. All cells have 2 to 3 marker chromosomes with terminal centromeres.
Clinical Data
female
Virus Susceptibility Vesicular stomatitis virus
Rabies virus
Human poliovirus 3
Comments
The cells produce a C type retrovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 30.0°C
Name of Depositor HF Clark
Year of Origin 1967
References

Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059

Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139

Basic Documentation
Restrictions

This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit

References

Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059

Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139