Hs 294T (ATCC® HTB-140)

Organism: Homo sapiens, human  /  Cell Type: Melanoma  /  Tissue: skin; derived from metastatic site: lymph node  / 

Organism Homo sapiens, human
Tissue skin; derived from metastatic site: lymph node
Cell Type Melanoma
Product Format frozen
Morphology mixed stellate and polygonal
Culture Properties adherent
Biosafety Level 1
Age 56 years
Gender male
Ethnicity Caucasian
Karyotype (P26) heteroploid with modal number = 59; Y chromosomes present
Derivation

ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue.


Clinical Data
56 years
Caucasian
male
Receptor Expression
Nerve growth factor (NGF); interferon
Tumorigenic Yes
Effects
Yes, in immunosuppressed NAMRU mice
Comments

The proliferative rate of the cell line is enhanced by addition of retinoic acid.

Responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon.

Results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week

Cryopreservation
Culture medium, 95%; DMSO, 5%
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
Me-2, 2
PGM1, 1-2
PGM3, 1
Name of Depositor RB Owens
References

Czarniecki CW, et al. Synergistic antiviral and anti-proliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. J. Virol. 49: 490-496, 1984. PubMed: 6319748

Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934

Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204

Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522

Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563

Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741

Cross References

Nucleotide (GenBank) : U02329 Human clone ndf294 neu differentiation factor mRNA, partial cds.

Basic Documentation
References

Czarniecki CW, et al. Synergistic antiviral and anti-proliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. J. Virol. 49: 490-496, 1984. PubMed: 6319748

Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934

Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204

Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522

Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563

Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741