Hs 929.Sk (ATCC® CRL-7681)

Organism: Homo sapiens, human  /  Disease: Granuloma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Product Format flask
Morphology mixed
Culture Properties adherent
Biosafety Level 1
Disease Granuloma
Age 5 years
Gender male
Ethnicity Caucasian
Applications
The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682).
This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 46
Images
Derivation
The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682).
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Clinical Data
The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682).
male
Caucasian
5 years
Comments
The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682).
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Note: This item is distributed only within the 50 United States. It is not available for international distribution.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Passage History
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation