Hs 53.T (ATCC® CRL-7033™) Organism: Homo sapiens, human / Disease: neurofibroma General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Product Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Disease neurofibroma Age 13 years adolescent Gender female Ethnicity Black Storage Conditions liquid nitrogen vapor phase Karyotype modal number = 45; range = 34 to 47 Clinical Data femaleBlack13 years adolescent Comments Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.Subcultivation Ratio: 1:2 to 1:3 Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5% Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°C STR Profile Amelogenin: XCSF1PO: 7,10D13S317: 9,12D16S539: 10,12D5S818: 12,13D7S820: 12THO1: 8,9TPOX: 7,9vWA: 15,16 Year of Origin January 31, 1969 References The line was derived from non-neoplastic tumor from the floor of the mouth. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS References The line was derived from non-neoplastic tumor from the floor of the mouth.