CCD-1136Sk (ATCC® CRL-2697)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 36 years
Gender male
Applications
The cell line was derived from normal skin taken from the leg (below the knee) of a male who died in a motorcycle accident.
Derivation
The cell line was derived from normal skin taken from the leg (below the knee) of a male who died in a motorcycle accident.
Clinical Data
The cell line was derived from normal skin taken from the leg (below the knee) of a male who died in a motorcycle accident.
male
Comments
The cell line was derived from normal skin taken from the leg (below the knee) of a male who died in a motorcycle accident.
Cells senesce after approximately 42 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor F.Haq, ATCC