CCD-1129SK (ATCC® CRL-2575)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Carcinoma,Cancer

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinoma,Cancer
Age 46 years
Gender female
Applications
The line was established from skin taken from normal breast tissue removed at bilateral mastectomy.
Derivation
The line was established from skin taken from normal breast tissue removed at bilateral mastectomy.
Clinical Data
The patient was diagnosed with grade II/III infiltrating ductal carcinoma of the left breast and high grade ductal carcinoma in situ of the right breast.
The tissue donor had a strong family history of breast cancer.
female
Comments
The line was established from skin taken from normal breast tissue removed at bilateral mastectomy.
The patient was diagnosed with grade II/III infiltrating ductal carcinoma of the left breast and high grade ductal carcinoma in situ of the right breast.
The tissue donor had a strong family history of breast cancer.
Cells senesce after approximately 36 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor A Thompson
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation