CCD 1102 KERTr (ATCC® CRL-2310)

Organism: Homo sapiens, human  /  Cell Type: keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transforme  / 

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Organism Homo sapiens, human
Cell Type keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transforme
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells may contain the human papilloma viral (HPV) sequences
Age 112 days gestation fetus
Applications
6E7 sequences were detected by PCR in cells at passage 18.Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Storage Conditions liquid nitrogen vapor phase
Karyotype hyperdiploid; about 55% of cells contain 45 to 50+ chromosomes
Derivation
Rockville Maryland, United States
Antigen Expression
epithelial specific antigen; Homo sapiens, expressed (Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155).)
Oncogene E6/E7 +
Genes Expressed
E6/E7 +,epithelial specific antigen; Homo sapiens, expressed (Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155).)
Comments
After 50 population doublings, the cells continue dividing and retain cuboidal morphology.
E6E7 sequences were detected by PCR in cells at passage 18.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Complete Growth Medium These cells are grown in Keratinocyte-Serum Free Medium (Gibco 17005-042)supplemented with
  • 0.05 mg/ml bovine pituitary extract (BPE)
  • 35 ng/ml human recombinant epidermal growth factor (EGF)
Do not filter complete medium.
Subculturing
Protocol: Remove medium, rinse two times with cold 0.25% trypsin, 0.53 mM EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach. Neutralize the trypsin with fresh culture medium, centrifuge, resuspend cells in fresh culture medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add fresh medium twice per week
Cryopreservation
Freeze medium: Ham's F12K medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isoenzymes
G6PD, A-B
Name of Depositor L Vilner, A Thompson
Passage History
After 50 population doublings, the cells continue dividing and retain cuboidal morphology.
E6E7 sequences were detected by PCR in cells at passage 18.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Year of Origin November, 1995
References

Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.

Fetal skin was digested with a collagenase-trypsin mixture. The digestion products were plated on collagen- fibronectin-BSA coated flasks in Keratinocyte Serum-Free Medium with epidermal growth factor and bovine pituitary extract. Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.

This lines has a rare human G6PD phenotype (A-B).

Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed for research purposes only. The American Type Culture Collection ( ATCC ) releases the line subject to the following: 1. CCD 1102 KERTr cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of the ATCC; 2. Any proposed commercial use of this cell line must first be negotiated with the ATCC, P.O. Box 1549, Manassas VA 20108, telephone 703-365-2704, FAX 703-365-2730.

References

Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.

Fetal skin was digested with a collagenase-trypsin mixture. The digestion products were plated on collagen- fibronectin-BSA coated flasks in Keratinocyte Serum-Free Medium with epidermal growth factor and bovine pituitary extract. Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.

This lines has a rare human G6PD phenotype (A-B).

Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155).