CCD-1094Sk (ATCC® CRL-2120)

Organism: Homo sapiens, human  /  Disease: Carcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Carcinoma
Age 57 years
Gender female
Ethnicity Caucasian
Applications
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
Derivation
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
Clinical Data
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
female
Caucasian
57 years
Comments
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
The cells senesce after approximately 41 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation