CCD-1074Sk (ATCC® CRL-2090)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 42 years
Gender female
Applications
The line was established from skin taken from normal breast tissue removed at mastectomy.
Derivation
The line was established from skin taken from normal breast tissue removed at mastectomy.
Clinical Data
female
Comments
The line was established from skin taken from normal breast tissue removed at mastectomy.
Cells senesce after approximately 26 population doublings.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%