CCD-1069Sk (ATCC® CRL-2089)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Carcinoma

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinoma
Age 70 years
Gender female
Applications
The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma.
Derivation
The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma.
Clinical Data
female
Comments
The line was established from skin taken from normal breast tissue removed at mastectomy for invasive ductal carcinoma.
Cells senesce after approximately 23 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%