CCD-944Sk (ATCC® CRL-1836)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 66 years
Gender female
Ethnicity Caucasian
Applications
The line was established from skin taken from the abdomen.
Derivation
The line was established from skin taken from the abdomen.
Clinical Data
female
Caucasian
66 years
Comments
The line was established from skin taken from the abdomen.
Cells from the current passage (2) have experienced about 14 population doublings beyond the biopsy material Subculture every 6 to 8 days.
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate; 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 12
D5S818: 10,11
D7S820: 7,10
THO1: 9,9.3
TPOX: 8
vWA: 16,18
Passage History
Cells from the current passage (2) have experienced about 14 population doublings beyond the biopsy material Subculture every 6 to 8 days.