CCD-34Sk (ATCC® CRL-1497)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 2.5 weeks
Gender female
Ethnicity Black
Applications
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility.
A normal lung line derived form the same patient is available as ATCC CRL-1491.
Derivation
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility.
A normal lung line derived form the same patient is available as ATCC CRL-1491.
Clinical Data
A normal lung line derived form the same patient is available as ATCC CRL-1491.
The child was delivered at 34 weeks gestation.
female
Black
2.5 weeks
Comments
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility.
A normal lung line derived form the same patient is available as ATCC CRL-1491.
The child was delivered at 34 weeks gestation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,13
D16S539: 8,13
D5S818: 12
D7S820: 10
THO1: 7,9
TPOX: 11
vWA: 15,16
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation