Detroit 539 (ATCC® CCL-84)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
Caucasian
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, sodium pyruvate (1 mM) and 0.1% lactalbumin hydrolysate, 90%; fetal bovine serum, 10%. Maintain pH at 7.3 to 7.4
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete medium 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation